Anti-Myosin light chain (phospho S20) antibody ab2480 from Abcam

Product Specs
ItemAnti-Myosin light chain (phospho S20) antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab2480
Antibody TypePrimary
Quantity50 UG
ClonalityPolyclonal
IsotypeIgG
HostRabbit
ApplicationsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/TagUnconjugated
FormatThis antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide
Concentration1 mg/ml
ModificationsPhospho-S20
Gene NameMYL9
ReactivityHuman, Mouse, Recombinant fragment
TargetMyosin light chain

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) 3D cell segregation geometry and dynamics are governed by tissue surface tension regulationCommun BiolAugust 4, 2023Elod Méhes, Enys Mones, Máté Varga, Áron Zsigmond, Beáta Biri-Kovács, László Nyitray, Vanessa Barone, Gabriel Krens, Carl-Philipp Heisenberg, Tamás Vicsekagainst phosphorylated (pS19) myosin light chain (rabbit, polyclonal, Anti-MYL12A phospho S19, Abcam ab2480) was applied in 1/100 dilution for 2 h at room temperature and then overnight at 4 °C. Anti-rabbit secondary

(2) Sandwich-like electro-conductive polyurethane-based gelatin/soybean oil nanofibrous scaffolds with a targeted release of simvastatin for cardiac tissue engineeringJournal of Biological EngineeringJuly 6, 2023Solmaz Saghebasl, Abbas Nobakht, Hesam Saghebasl, Sanya Hayati, Ozra Naturi, Reza Rahbarghaziwas continued by the transfer of samples to PVDF membranes and incubated with anti-myosin (Cat No: ab2480; Abcam), anti-Connexin-43 (Cat no: sc-13,558; San Cruz Biotech. Inc.), anti-α-actinin (Cat no: sc-130,928;

(3) Internalization of apoptotic cells during efferocytosis requires Mertk-mediated calcium influxCell death & diseaseJune 30, 2023Yang S, Min C, Moon H, Moon B, Lee J, Jeon J, Kwon H, Jang D, Park D.Rac1 Activation Assay Kit (17–283; Merck, Darmstadt, Germany). The antibodies used in this study were anti-pS20 MLC (ab2480; Abcam, Cambridge, UK), anti-pT18/pS19 MLC (3674 S; Cell Signaling Technology, Danvers, MA, USA), anti-MLCK

(4) Vascular mechanisms of post-COVID-19 conditions: Rho-kinase is a novel target for therapyEuropean Heart Journal - Cardiovascular PharmacotherapyJune 2, 2023Robert A Sykes, Karla B Neves, Rhéure Alves-Lopes, Ilaria Caputo, Kirsty Fallon, Nigel B Jamieson, Anna Kamdar, Assya Legrini, Holly Leslie, Alasdair McIntosh, et al.Rho-kinase in this cohort, with staining for phosphorylated myosin light chain (pMLC) antibodies (Abcam ab2480). pMLC phosphorylation reflects enhanced sensitivity towards Ca2+ linking actin–myosin filaments for

(5) Biophysical forces mediated by respiration maintain lung alveolar epithelial cell fateCellMarch 30, 2023Shiraishi K, Shah PP, Morley MP, Loebel C, Santini GT, Katzen J, Basil MC, Lin SM, Planer JD, Cantu E, et al.MAB1179, 1:100), Krt8 (rat, DSHB, TROMA-1, 1:200), Phosphorylated Myosin Light Chain (rabbit, Abcam, ab2480, 1:100), FAK-pY397 (rabbit, Thermo, 44–624G, 1:100), Yap (rabbit, Cell Signaling, 4912S, 1:100), Lamp3

(6) Wnt4 and ephrinB2 instruct apical constriction via Dishevelled and non-canonical signalingNature communicationsJanuary 20, 2023Yoon J, Sun J, Lee M, Hwang YS, Daar IO.anti-C-cadherin (1:50, 6B6, DSHB), Mouse anti-ZO1 (1:250, ZO1-1A12, ThermoFisher), Rabbit anti-pMLC (1:250, ab2480, Abcam), Mouse anti-HA-Alexa Fluor-488/555/647 (1:500, 2-2.2.14, ThermoFisher), Mouse anti-V5-Alexa

(7) An entosis-like process induces mitotic disruption in Pals1 microcephaly pathogenesisNature communicationsJanuary 5, 2023Sterling NA, Park JY, Park R, Cho SH, Kim S.al., 2012), PAX6 (1:200; Biolegend, 901301), PH3 (1:500; Millipore Sigma, H0412), pMLC (1:200; Abcam, ab2480), MPM2 (1:200; Cell Signaling, 05-368), SURVIVIN (1:200; Cell Signaling, 2808), TBR2 (1:200; Abcam,

(8) Androgen-regulated MafB drives cell migration via MMP11-dependent extracellular matrix remodeling in miceiScienceDecember 22, 2022Alcantara MC, Suzuki K, Acebedo AR, Kajioka D, Hirohata S, Kaisho T, Hatano Y, Yamagata K, Takahashi S, Yamada G.ab150361,Abcam,Cat. No.: ab150361; RRID:AB_2631309 Myosin light chain (phospho S20) antibody,Abcam,Cat. No.: ab2480; RRID:AB_303094 Phospho-FAK (Tyr397) Recombinant Rabbit Monoclonal Antibody (31H5L17),Thermo Fisher Scientific

(9) Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degenerationiScienceOctober 21, 2022Tian H, Chen Z, Zhu X, Ou Q, Wang Z, Wu B, Xu JY, Jin C, Gao F, Wang J, et al.N/A" MERTK,Abcam,"ab52968, RRID:AB_2143584" Rhodopsin,Abcam,"ab98887, RRID:AB_10696805" MYOSIN,Abcam,"ab2480, RRID:AB_303094" α-SMA,Abcam,"ab5694, RRID:AB_2223021" FOXF2,Abcam,"ab54993, RRID:AB_2105608" SMAD2,Abcam,"ab33875,

(10) Extracellular Hydraulic Resistance Enhances Cell MigrationAdvanced science (Weinheim, Baden-Wurttemberg, Germany)October 1, 2022Maity D, Bera K, Li Y, Ge Z, Ni Q, Konstantopoulos K, Sun SX.was used. For staining of pMLC, 1:2000 Primary: Anti‐Myosin light chain (phospho S20) antibody (Abcam ab2480) and 1:250 Secondary: Goat Anti‐Rabbit IgG H&L (Alexa Fluor 488) (Abcam ab150077) volumetric ratio in

(11) Tumor cell-conditioned media drives collagen remodeling via fibroblast and pericyte activation in an in vitro premetastatic niche modeliScienceJuly 15, 2022Nairon KG, DePalma TJ, Zent JM, Leight JL, Skardal A.Blocker BSA. Slides were incubated overnight at 4 °C in a 1:200 dilution of anti-myosin light chain (ab2480, Abcam) and anti-paxillin (AHO0492, Thermo Fisher Scientific) in 1% BSA, followed by a 1-h incubation

(12) Developmentally interdependent stretcher-compressor relationship between the embryonic brain and the surrounding scalp in the preosteogenic headDevelopmental dynamics : an official publication of the American Association of AnatomistsJuly 1, 2022Tsujikawa K, Saito K, Nagasaka A, Miyata T.10 (mouse, ab9025; Abcam, 1:500), anti‐elastin (rabbit, ab21610; Abcam, 1:500), anti‐pMLC (rabbit, ab2480; Abcam, 1:500), anti‐Runx2 (rabbit, #12556; Cell Signaling, 1:1600), anti‐αSMA (rabbit, 23 081‐1‐AP;

(13) Dysregulation of the Enteric Nervous System in the Mid Colon of Complement Component 3 Knockout Mice with Constipation PhenotypesInternational Journal of Molecular SciencesJune 20, 2022Yun Ju Choi, Hee Jin Song, Ji Eun Kim, Su Jin Lee, You Jeong Jin, Yu Jeong Roh, Ayun Seol, Hye Sung Kim, Dae Youn Hwanganti-p-PI3K (Cell Signaling Technology Inc.; #4228), anti-MLC (Abcam Com.; ab79935), anti-p-MLC (Abcam Com.; ab2480), anti-nNOS (Abcam Com.; ab3511), anti-NSE (Abcam Com.; ab79757), anti-C-kit (DAKO, Kyoto, Japan; A4502),

(14) Pericyte-mediated constriction of renal capillaries evokes no-reflow and kidney injury following ischaemiaeLifeMarch 14, 2022Freitas F, Attwell D.(1:100; Abcam ab5694, Cambridge, United Kingdom), or myosin light chain (phospho S20, 1:100, Abcam ab2480, Cambridge, United Kingdom), and the capillary basement membrane and pericytes were labelledwith isolectin

(15) IP3 receptor orchestrates maladaptive vascular responses in heart failureThe Journal of clinical investigationFebruary 15, 2022Dridi H, Santulli G, Gambardella J, Jankauskas SS, Yuan Q, Yang J, Reiken S, Wang X, Wronska A, Liu X, et al.Santa Cruz Biotechnology); anti-IP3R3 (610312 , BD Biosciences); anti–phosphorylated MLC20 (anti–p-MLC20) (ab2480, Abcam), anti-MLC20 (ab137063, Abcam); anti-RyR2 (ab55999, Abcam); anti–p-RyR2 (ab59225, Abcam); and

(16) Anti-Cancer Effects of α-Cubebenoate Derived from Schisandra chinensis in CT26 Colon Cancer CellsMoleculesJanuary 23, 2022Jeong Eun Gong, Ji Eun Kim, Su Jin Lee, Yun Ju Choi, You Jeong Jin, Young Whan Choi, Sun Il Choi, Dae Youn Hwang(Cat. No. 3283S, Cell Signaling Technology), anti-MLC (Cat. No. ab92721, Abcam), anti-p-MLC (Cat. No. ab2480, Abcam), or anti-β-actin antibodies (Cat. No. 4967S, Cell Signaling Technology)} overnight at 4°C. Probed

(17) Agrin-Matrix Metalloproteinase-12 axis confers a mechanically competent microenvironment in skin wound healingNature communicationsNovember 3, 2021Chakraborty S, Sampath D, Yu Lin MO, Bilton M, Huang CK, Nai MH, Njah K, Goy PA, Wang CC, Guccione E, et al.Technologies, Antibody #3671, RRID:AB_330248 IF dilution 1:50, Anti-Myosin light chain (phospho-S20) antibody (ab2480), RRID:AB_303094 WB Dilution 1:1000; Myosin Light Chain 2 Antibody #3672, RRID: AB_330278 WB Dilution

(18) Adaptations in Hippo-Yap signaling and myofibroblast fate underlie scar-free ear appendage wound healing in spiny miceDevelopmental CellOctober 4, 2021Chris M. Brewer, Branden R. Nelson, Paul Wakenight, Sarah J. Collins, Daryl M. Okamura, Xiu Rong Dong, William M. Mahoney, Aaron McKenna, Jay Shendure, Andrew Timms, et al.(M2),Sigma-Aldrich,"Cat# F1804, RRID: AB_262044" Rabbit polyclonal anti-Myosin Light Chain (phospho S20),Abcam,Cat# ab2480 RRID: AB_303094 Rabbit polyclonal anti-Phospho-LATS1 (Thr1079),Cell Signaling Technologies,"Cat# 9159S,

(19) Otic Neurogenesis in Xenopus laevis: Proliferation, Differentiation, and the Role of Eya1Frontiers in NeuroanatomySeptember 20, 2021Suad Hamdan Almasoudi, Gerhard SchlosserT6793",Mouse-Monoclonal,1:1000 N-cadherin (CDH2),"Abnova, PAB7876",Mouse-Monoclonal,1:200 Myosin light chain (phosph S20),"Abcam, ab2480",Rabbit-Polyclonal,1:200 Atypical protein kinase (aPKC),"Santa Cruz Biotechnology, sc-216",Rabbit-Polyclonal,1:200 Partitioning-defective

(20) SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injuryiScienceMay 21, 2021Getachew A, Abbas N, You K, Yang Z, Hussain M, Huang X, Cheng Z, Tan S, Tao J, Yu X, et al.antibody,Proteintech,Cat#21173-1-AP; RRID:Addgene_80089 Anti-Myosin light chain (phospho S20) antibody (ab2480),Abcam,Cat#ab2480; RRID:Addgene_80089 Anti-TLR4 antibody (ab13556),Abcam,Cat# ab13556; RRID:AB_300457 AGER

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Anti-Myosin light chain (phospho S20) antibody from Abcam

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Anti-Myosin light chain (phospho S20) antibody from Abcam

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